Journal: Cell reports

Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

doi: 10.1016/j.celrep.2025.115374

Figure Lengend Snippet: (A–D) GLUA2 protein level is decreased at intracortical synapses in Gpc5 cKO mice at P28. (A) Immunostaining of intracortical presynaptic VGLUT1 and postsynaptic GLUA2 in L2/3. (B–D) Numbers of VGLUT1 (B), GLUA2 (C), and colocalized (D) puncta. N = 5 mice/condition. (E–H) GLUA1 protein level is unchanged at intracortical synapses in Gpc5 cKO mice at P28. (E) Immunostaining of presynaptic VGLUT1 and postsynaptic GLUA1 in L2/3. (F–H) Numbers of VGLUT1 (F), GLUA1 (G), and colocalized (H) puncta. N = 6 mice/condition. (I–P) Thalamocortical synapses have unaltered AMPAR levels in Gpc5 cKO mice at P28. (I) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA2 in L4. (J–L) Numbers of VGLUT2 (J), GLUA2 (K), and colocalized (L) puncta. N = 5 mice/condition. (M) Immunostaining of thalamocortical presynaptic VGLUT2 and postsynaptic GLUA1 in L4. (N–P) Numbers of VGLUT2 (N), GLUA1 (O), and colocalized (P) puncta. N = 5 mice/condition. (Q–S) VGLUT2 puncta volume is decreased in P28 Gpc5 cKO mice. (Q) Immunostaining of VGLUT2 puncta in L1 VC. (R and S) VGLUT2 puncta volume in L1 and L4. N = 6 mice/condition L1, N = 5 mice/condition L4. (T) Summary of synaptic changes in Gpc5 cKO mice at P28. Scale bars: 5 μm (A, E, I, M, and Q, main) and 1 μm (A, E, I, and M, magnified). Graphs: mean ± SEM. Individual data points represent mice. Statistics: t test. See also .

Article Snippet: Rabbit polyclonal anti GluA1 , Millipore , CAT# AB1504; RRID:AB_2113602.

Techniques: Immunostaining

Journal: Cell reports

Article Title: Astrocyte glypican 5 regulates synapse maturation and stabilization

doi: 10.1016/j.celrep.2025.115374

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti GluA1 , Millipore , CAT# AB1504; RRID:AB_2113602.

Techniques: Recombinant, Modification, Saline, RNAscope, Multiplex Assay, Electron Microscopy, Negative Control, Software, Cell Culture, Fluorescence, Microscopy, Hybridization

Journal: iScience

Article Title: Disuse plasticity limits spinal cord injury recovery

doi: 10.1016/j.isci.2025.112180

Figure Lengend Snippet:

Article Snippet: Polyacrylamide gel electrophoresis was performed by loading 10 μg of protein per sample into an individual lane of a precast 10-20% Tris-HCl polyacrylamide gel with three independent replications according to a randomized counterbalancing fashion blindly., Protein was transferred to a nitrocellulose membrane, blocked, and probed with primary antibodies: rabbit polyclonal anti-GluA1 (1:200; Millipore, Billerica, MA; Cat# AB1504, RRID: AB_2113602 ), rabbit polyclonal anti-GluA2 (1:200; Millipore; AB1768-25UG, RRID: AB_2247874 ), rabbit monoclonal anti-pS831-GluA1 (1:200; Millipore; Cat# 04-823, RRID: AB_1977218 ), rabbit polyclonal anti-pS880-GluA2 (1:200; Millipore; Cat# 07-294, RRID: AB_568822 ), and mouse monoclonal anti-β-Actin as a loading control (1:1500; BD Biosciences; Cat# 612657, RRID: AB_399901 ) in blocking buffer.

Techniques: Recombinant, Protease Inhibitor, Blocking Assay, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Imaging, Inverted Microscopy, Microscopy